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Composite

Part:BBa_K1420000:Design

Designed by: Jessica Tarnowski   Group: iGEM14_Minnesota   (2014-10-08)


Mer operon, biological system found to detoxify organic and inorganic forms of mercury


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 551
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2420
    Illegal NgoMIV site found at 2468
    Illegal NgoMIV site found at 2530
    Illegal NgoMIV site found at 2741
    Illegal NgoMIV site found at 3334
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 536


Design Notes

The original plasmid obtained from A. O. Summer's lab contained a the EcoRI restriction site within the operon. Mutagenesis by the UMN iGEM team removed this internal restriction site.


Source

This part was acquired courtesy of A. O. Summer's lab, in the pDU1358 plasmid.

References